WebNational Center for Biotechnology Information WebFeb 6, 2024 · PROCEDURE. After exactly 7 minutes of incubation, add 2.0 mL of iodide solution to each tube. Add 30 μ L of appropriate control or sample to each “blank” tube. Transfer the contents of each 13 x 100 mm tube to the corresponding 19 x …
Experiment-12 Estimation of Serum Uric Acid by Caraway’s Method
WebBlauch and Koch Method First Step (Uricase) · Principle: Enzymatic production of allantoic from uric acid · In the presence of uricase/urate oxidase, uric acid is converted to … Web3 Coupled enzyme (II) Peroxidase H 2 O 2 + indicator dye colored compound Chemical Method Principle Phosphotungstic acid (Caraway method) Uric Acid + H 3 PW 12 O 4o + O 2 - Na 2 CO 3 /OH- allantoin + tungsten blue + CO 2 IV. Specimen Requirement SPECIMEN CONSIDERATIONS 1. May be measured using heparinized plasma, serum … fnb life health cash plan
Uric Acid measurement Flashcards Quizlet
WebPrinciple : Uric acid is oxidized to allantion and carbon dioxide by a phosphotungstic . acid regent in alkaline solution . Phosphotungstic acid is reduced in this reaction to tungsten blue . which is measured at 710 nm. Protein have been removed by precipitation with tungstic acid , TCA , phosphotungstic acid and heat coagulation . WebDec 14, 2024 · Principle: Uricase converts uric acid to allantoin and hydrogen peroxide. The hydrogen peroxide formed further reacts with a … WebJul 8, 2024 · method principles, sample and reagent volumes, analysis time, cost and precision, is available (in German) in the review of Gihitz.12 AMYI.OCI.ASTIC METHODS The amyloclastic methods measure the break down of a starch substrate hy viscosirnetric, turbidimetric, iodornetric or nephelometric procedures. Yiscosimctric green tech bio products