2024 Rag2-gfp mice

Rag2-gfp mice

Author: ixkg

August undefined, 2024

005688 FVB-Tg(Rag2-EGFP)1Mnz/J These NG-BAC transgenic mice express enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter, and may be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression. WebThese mice may be useful in the study of hematopoietic and immune system defects, cancer, toxicology, and xenograft/transplant studies. Of note, a C57BL/6J isogenic Rag2 …

Thymic development of gut-microbiota-specific T cells Nature

WebApr 15, 2024 · Cx3cr1 deficiency exacerbates glomerulonephritis in female MRL/lpr mice. To directly elucidate the role of CX 3 CR1 + cells in spontaneous SLE, we backcrossed the Cx3cr1-gfp/gfp locus from B6 background (The Jackson Laboratory/JAX Stock Number 005582) to lupus-prone MRL/lpr mice (JAX Stock Number 000485). The Cx3cr1-gfp/gfp … Webhomozygous (Rorγtgfp/gfp) RORγt-reporter mice. In the lamina propria of homozygous (knock-out) mice the complete disappearance of CD4hiGFP+ and CD4-GFP+ cryptopatch or lymphoid tissue inducer-like cells is evident. ... Lymphocytes were isolated from the spinal cords of Rag2-/- mice reconstituted with CD4+ internships creative writing

Autoreactive thymic B cells are efficient antigen-presenting cells …

WebAug 13, 2024 · To identify iNKT cells at an early stage of development in the thymus, we used mice that express green fluorescent protein (GFP) under the control of the recombination-activating gene 2 (Rag2) promoter via a bacterial artificial chromosome transgene (Rag2 GFP), in which GFP expression indicates the ‘age’ of cells (Boursalian et … WebRFP mice splenocytes stained with B220 APC and CD8 APC (down) ( B ) Representative FACS plots of post-depletion samples composed of a mix of B220-, CD8-, IL18R- depleted Foxp3. GFP splenocytes and B220-, CD8- depleted Foxp3. RFP splenocytes. Numbers indicate the percentage of cells within each gate. Techniques Used: Staining Figure … WebJul 25, 2011 · (F) Adoptive transfer of control or mCD47/BALB-HIS spleen T cells into adult, nonmanipulated BALB/c Rag2 −/− IL-2Rγc −/− mice. The number of human GFP + T cells recovered from the spleen of the recipient mice is plotted as a percentage of recovery from the initial inoculum (n = 4 control vs. n = 3 mCD47 per time point). internship sde

SRSF1 serves as a critical posttranscriptional regulator at the late ...

Male-Dependent Promotion of Colitis in 129 Rag2−/− Mice Co …

WebMay 12, 2024 · To confirm that these cells were thymocytes, we colonized mice expressing green fluorescent protein (GFP) from the RAG2 promoter (hereafter, RAG2-GFP mice) 14 … WebIn the thymus and bone marrow of heterozygous RAG2-GFP mice, as expected, GFP expression was detected in the appropriate stages of developing T and B cells. … new economy airlineWeb(B) FACS analysis of peripheral blood of representative recipient mice at 20 wk (N; AG) and 13 wk (PG) post-transplantation. The fluorescence intensity of GFP (marking ES cell-derived cells) is indicated on the X -axis, the fluorescence intensity of differentiation markers (myeloid: Gr-1; T-cell: CD4/CD8) is indicated on the Y -axis. new economy business park

"WebJan 19, 2005 · We used mice with a functional RAG2/GFP fusion gene inserted into the endogenous RAG2 locus ( 21) that allows for tracking of RAG2-expressing cells by their … " - Rag2-gfp mice

Rag2-gfp mice

Coordinate Regulation of RAG1 and RAG2 by Cell Type-Specific

WebJul 12, 2024 · C57BL/6 mice were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China. NG-BAC (RAG2-GFP) transgenic reporter mice were as described (Yu et al., 1999). All animal experiments were performed under animal ethical regulations, and the study protocol was approved by the Institutional Animal Care and Use … WebApr 14, 2024 · In order to determine the effect of the immune system on disease progression, 1 o AMLs were transplanted into secondary (2 o) non-irradiated immunodeficient Rag2 −/− γc −/− or ...

Did you know?

WebMay 30, 2006 · We recently used animals (14) carrying a GFP transgene driven by the recombination-activating gene 2 (RAG2) promoter [RAG2p-GFP transgenic (Tg) mice] to … WebOT-II Rag2 Transgenic Knockout Mouse Taconic Biosciences Rag2/OT-II Constitutive Knockout / Random Transgenic Inquire CALL: 888.822.6642 + 45 70 23 04 05 (EUROPE) …

WebIn the thymus and bone marrow of heterozygous RAG2-GFP mice, as expected, GFP expression was detected in the appropriate stages of developing T and B cells. Interestingly, only a fraction of Thy-1.2 (+) cells in the Peyer's patch were found to be GFP (+) amongst the peripheral lymphoid organs. WebTg (Rag2-EGFP)1Mnz. Mutation details : A mouse BAC clone Containing Rag1 and Rag2 was obtained from Research Genetics. An EGFP cDNA was inserted in this clone at the start …

WebMice which lack Rag2 and Il2rg do not develop functional T, B or NK cells. Analysis of spleen cells showed no detectable NK1.1+ cells, B220+ B cells or Thy1+ T cells. A small number … WebAug 1, 1999 · RAG2:GFP expression is undetectable in most immature splenic B cells; however, in young RAG2:GFP mice, there are substantial numbers of splenic RAG2:GFP + …

WebMay 5, 2009 · A, Single cell suspensions were prepared from Lymph nodes (LNs) from naive either RAG2 -/- or IL-23R-GFP.KI RAG2 -/- mice and IL-23R(GFP) expression was analyzed on total LNs' cells. B, IL-23R-GFP.KI mice were immunized with MOG 35-55 /CFA. On day 17, IL-23R(GFP) expression was analyzed on myeloid cell populations as indicated in histogram.

WebRFP mice splenocytes stained with B220 APC and CD8 APC (down) ( B ) Representative FACS plots of post-depletion samples composed of a mix of B220-, CD8-, IL18R- depleted … new economy bookWebThese grafts are not rejected in this mouse strain because the Rag2 −/− γ c −/− mice are immunodeficient, lacking NK, T and B cells. The recovered FTOC-T cells were subcutaneously infused around the skin graft ( Figure 5D ) 4 weeks posttransplantation to determine if the T cells recognized and responded to the allogenic MRL skin grafts. new economy boardWebTo visualize developing thymic B cells, we used the Rag2-GFP transgenic mice . GFP expression marks cells that are actively undergoing either TCR or BCR rearrangement, and due to the relatively long half-life of GFP protein, low levels persist in cells after its expression has been turned off, marking cells that have recently expressed Rag . To ... internships dolWebRag2p Gfp Mouse, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … internships department of defenseWebTCRβ + CD4 + and CD8 + T cells from spleen, LNs, and PBCs of Rag2GFPSrsf1fl/flLckCre/+ and littermate Rag2GFP (as control) mice were measured by flow cytometry. Representative histograms show the percentages of GFP + cells from indicated populations. new economy boomWebJan 24, 2024 · Rag2 Targeted Allele Detail MGI Mouse (MGI:3623131) Search All Search Tools Genes Genes & Markers Query Batch Query JBrowse Genome Browser … internships dodWebThese NG-BAC transgenic mice express enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter, and may be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression. #005688 WHAT TO EXPECT A confirmation will be sent after order placement. Typically, eight or more animals are produced. new economy business park ambridge