Spoton flow cell
WebSpotON Flow Cell Rev D (R9.4) Suitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire … WebLoading a Flow cell in 3 steps 1. check for small bubble under the cover. Draw back a small volume to remove any bubble (a few µls) 2. Prime the Flow Cell a. Load 800 µl of the …
Spoton flow cell
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Web17 May 2024 · DNA libraries were sequenced using a version 2 300-cycle kit (2 × 150-bp paired-end reads) on an Illumina MiSeq platform. Genomic DNA was also processed using end repair and A-tailing reagents (New England BioLabs) and the 1D ligation sequencing kit MinION Mk1B with the SpotON flow cell R9.4 (Oxford Nanopore Technologies). WebDirect RNA sequencing was performed using a MinION device, and one v9.4 SpotON MinION flow cell (FLO-MIN106, Oxford Nanopore Technologies) per species was used. The produced reads were corrected ... The extension speed was applied at 10 mm/min, and the force during testing was measured with a 1-N load cell. The tensile strength, Young’s ...
WebComplete the flow cell priming: Gently lift the SpotON sample port cover to make the SpotON sample port accessible. Load 200 µl of the priming mix into the flow cell priming … Web1 Jul 2024 · For further validation of AmpliCoV sequencing, samples from patients diagnosed with SARS-CoV-2 by PCR were amplified, prepared with the Rapid Barcoding …
WebOur base pay range starts at $74,000 -$119,000 for this role. Please note the salary range listed is just one component of a competitive compensation package which includes a company stock plan. Offers will be reflective of the candidate's location and experience. SpotOn is an equal employment opportunity employer. WebIt is vital that the flow cell priming port and SpotON sample port are closed to prevent air from being drawn across the sensor array area, which would lead to a significant loss of …
Web4 Dec 2024 · The sequencing-ready libraries were loaded on R9.4 SpotON Flow Cells, and the Albacore v1.2.6 software was used for base calling. Table 6 Overview table of the …
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